pGLO Lab

1.

Plate	Number of Colonies	Color of colonies under room light	Color of colonies under UV  light
-pGLO LB	covered	brownish yellow	whitish blue
-pGLO LB/amp	0	N/A	N/A
+pGLO LB/amp	100~	brownish yellow	whitish blue
+pGLO LB/amp/ara	22	brownish yellow	glowing green

2. The bacteria from the +pGLO LB/amp/ara plate gained two new traits. The transformed bacteria are resistance to ampicillin and the ability to glow under UV light. They did not die in the presence of ampicillin, and glowed under the light.

3. Each transformed bacteria must have multiplied into a colony, so about 100 bacteria were transformed in the +pGLO LB/amp. The bacteria that did not pick up the plasmid would have been killed by the ampicillin. Probably half of the bacteria died in the plate, so probably over 250 bacteria were present in the 100 micro-liters.

4. The purpose of arabinose is to "turn on" the GFP or Green Fluorescent Protein. This causes the bacteria to glow green under UV light. 

5. There are several uses for GFP for biologists. It can be used as a marker when inserting a gene into a cell. If the cell glows, it means the other gene inserted is also being expressed. The protein has also been altered to react to different wavelengths and express several different colors. 

6. Another practical application of genetic engineering is in food production weather it be animals or crops. Genetic engineering can make an animal or plant stronger, bigger, or more resistant to disease. 

Source: https://embryo.asu.edu/pages/green-fluorescent-protein